Human Serum, sterile filtered
Product information "Human Serum, sterile filtered"
Since 1988, PAN-Biotech is known as a renowned producer of sera for cell culture.
Our customers benefit from our years of experience and highly specialized products.
From collection to the final product
The sample collection is taken from human, healthy donors in Europe, certified donations institutions.
Each donation is precisely tested regarding various biophysical parameters and viral infections and negative in the tests anti HIV 1/HIV 2, Hbs Ag, anti-HCV, HIV-1 Ag and/or HIV-1 NAT and Syphilis.
In each batch of human serum among others the osmolarity, pH, protein- and glucose concentration, and cell growth are measured and the sterility tested.
Human Sera from PAN-Biotech consist exclusively of human material. Human serum may be more suitable for the cultivation of human cells than FBS since serum and the cultured cells belong to the same species. Thus, the physiological and natural conditions in vitro are reproduced most similar to those in vivo. Human serum is particularly suitable for cell culture of human cells e.g. research in cell and immunotherapy, tissue engineering and other applications where the presence of animal derived components is unwanted.
PAN-Biotech offers three different categories of human serum: "Off the clot", Seraclot and converted human serum. Within these three categories it is possible to choose between a mixed groups pool or human serum AB.
Human Serum from PAN-Biotech
Human serum is produced from human plasma by addition of calcium chloride. This results in clotting of the plasma. After the coagulation process the cellular and solid constituents of the serum are removed by centrifugation, the human serum is washed and concentrated by ultra-filtration and finally filtered through a combination of depth- and membrane- filters.
PAN-Biotech offers also converted human serum from donors with the blood group AB. Their blood does not contain anti-A nor anti-B antibodies, that could damage cultured cells and is therefore particularly suitable for immunological and hematopoietic studies.
Customer information
Human serum is a natural product. After the sterile filtration it is bottled and frozen as a clear solution. After thawing a slight blur may occur. This does not mean any loss of quality and the serum can be added to the medium without concerns.
Human serum is usually used in a concentration between 5% and 20%.
Cells cultured with FBS should be adapted step by step to the new serum. Initially, the cells should be cultivated for 4-6 days with a 10% serum mixture, consisting of 5% FBS and 5% human serum. Afterwards the final conversion to human serum can take place.
| Blood Type: | unspecified |
|---|---|
| Country of Origin: | Country unspecified |
| Endotoxin Content: | Endotoxin Content unspecified |
| Filter Pore Size: | 0.2 µm |
| Humansera: | Normal |
| Liquid / Powder: | Liquid |
| Product Category: | Human Sera |
| Product Type: | Human Serum |
| Sex: | Unspecified |
| Size: | 100 ml |
| Species of Origin: | Human |
| Sterile: | Yes |
| Storage: | -20°C |
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