IVT Reaction Buffer 10x
€209.60
Product information "IVT Reaction Buffer 10x"
IVT Reaction Buffer 10x is a 10x concentrated reaction buffer for in-vitro transcription (IVT) of mRNA from a linearised DNA template. It provides the buffered environment for RNA-polymerase-driven, cell-free mRNA synthesis.
This GMP-grade buffer is manufactured by PAN-Celltech under EU GMP Annex 1 and released against a defined quality-control panel on every lot. As a 10x concentrate, it dilutes conveniently into the reaction.
These features make IVT Reaction Buffer 10x particularly suitable for cell-free mRNA synthesis in mRNA technology and cell and gene therapy, e.g. transient CAR mRNA and CRISPR guide RNA.
Typical Applications
- In-vitro transcription (IVT) of mRNA
- Synthesis of guide RNA (e.g. CRISPR gRNA)
- Transient CAR mRNA for cell and gene therapy
- Cell-free mRNA synthesis in mRNA technology
Product Features
- GMP grade, manufactured under EU GMP Annex 1
- 10x concentrate: Tris-HCl (pH 7.9), MgCl2, spermidine, DTT
- Clear, colourless to slightly yellowish solution
- Storage at -20 °C
- Note: adjust the Mg2+ concentration in the final IVT reaction to the NTPs used - keep total Mg2+ above total NTP
| Filter Pore Size: | 0.2 µm |
|---|---|
| Liquid / Powder: | Liquid |
| Quality: | GMP Excipient RNase - Free |
| Size: | 100 ml |
| Sterile: | Yes |
| Storage: | -20°C |