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PANiBoost, Human tubal fluid (HTF), w: NaHCO3

Product information "PANiBoost, Human tubal fluid (HTF), w: NaHCO3"

PANiBoost, with NaHCO3, is a modified Human Tubal Fluid medium (HTF) developed for scientific work with human sperm. In contrast to PANiPure SO-58250, the formulation contains 25 mM bicarbonate (NaHCO3).

Following supplementation with, for example, 3 mg/ml human serum albumin (HSA) or bovine serum albumin (BSA), PANiBoost enables the preparation, for example by swim-up or density gradient centrifugation, incubation, and investigation of sperm under capacitating conditions. PANiPureHSA(10%), our concentrated HSA stock solution (SO-58015), is particularly suitable for HSA supplementation of PANiBoost.

The formulation of PANiBoost was defined by researchers at the University of Münster as part of the ProCon.MS study ( https://www.medizin.uni-muenster.de/reproductionms/proconms.html ) in order to ensure standardized experimental conditions as well as comparable and reproducible results across different study centers.

Features

  • Modified Human Tubal Fluid medium (HTF)
  • Contains 25 mM NaHCO3
  • Free from protein sources, HSA/BSA-free
  • Defined conditions for controlled supplementation with HSA/BSA, as required for sperm capacitation

Intended Use

  • Sperm preparation
  • Suitable for sperm capacitation following protein supplementation
  • Recommended protein supplementation with PANiPureHSA(10%) (SO-58015)
  • Reproductive biology and andrology research
  • Not intended for use in medically assisted reproduction (MAR)
Properties "PANiBoost, Human tubal fluid (HTF), w: NaHCO3"
Bicarbonate: with Sodium Bicarbonate
Ca/Mg: without Ca/Mg
Endotoxin Content: Endotoxin Content unspecified
Liquid / Powder: Liquid
Size: 50 ml
Sterile: Yes
Storage: +2°C - +8°C
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