Panserin 411S, Basal Medium: RPMI 1640, Serum-free, w: L-Glutamine, w: Insulin human rec.
€68.00*
Available, delivery time: 2-3 days
Product information "Panserin 411S, Basal Medium: RPMI 1640, Serum-free, w: L-Glutamine, w: Insulin human rec."
Panserin 411S is a fully defined, complete ready-to-use medium for the serum-free cultivation of myeloid and lymphoid cells for cytological examination.
Composition
Based on RPMI 1640 medium, additional trace elements, albumin, cholesterol, soya lipids, vitamins and hormones are added.
Suitability
Panserin 411S is a serum-free complete medium for the cultivation of myeloid and lymphoid cells from peripheral blood or bone marrow. It is therefore suitable for a rapid expansion of blood cells in order to investigate leukemic diseases (ALL, AML, CLL, CML, MPN, MDS). The state of the art diagnostic techniques of leukemic diseases are based on the interaction of cytomorphology including cytochemistry with immunophenotyping, chromosome banding analysis, FISH and molecular genetics. In Panserin 411S the number and quality of metaphases are significantly higher and independent of individual batches as compared to serum-containing media.
Application
1x107 cells are seeded in 5 ml Panserin 411S. Depending on the question or quality of the starting material, an unstimulated culture and another 1-3 cultures with appropriate growth factors are set up. The culture duration is 24 to 72 hours at 37°C in an incubator with 5% CO2 gassing. Metaphases are processed using hypotonic KCl solution and Carnoy's fixative.
| Size: | 500 ml |
|---|---|
| Storage: | +2°C - +8°C |