Skip to main content Skip to search Skip to main navigation

Universal Electroporation / Nucleofection Buffer

Product information "Universal Electroporation / Nucleofection Buffer"

The Universal Electroporation / Nucleofection Buffer is a defined buffer for electroporation- and nucleofection-based delivery of nucleic acids - plasmid DNA, mRNA or ribonucleoprotein (RNP) complexes - into mammalian cells.

It is designed for efficient, low-toxicity transfection of hard-to-transfect primary cells such as T cells, NK cells and stem cells. The GMP-grade buffer is manufactured by PAN-Celltech under EU GMP Annex 1, with every lot released against a defined quality-control panel.

These features make the buffer particularly suitable for non-viral, ex-vivo gene modification in cell and gene therapy workflows.

Typical Applications

  • Electroporation and nucleofection of plasmid DNA
  • Delivery of mRNA into primary cells
  • Delivery of RNP complexes
  • Non-viral gene modification of T cells, NK cells and stem cells
  • Ex-vivo gene modification in cell and gene therapy workflows

Product Features

  • GMP grade, manufactured under EU GMP Annex 1
  • Universal: for a broad range of primary and established cell types
  • Low-conductivity buffer with gentle, low-toxicity conditions to preserve cell viability
  • Phosphate buffer (pH 7.2) with KCl, MgCl2 and D-mannitol
  • Clear, colourless solution; storage at +2 to +8 °C
Properties "Universal Electroporation / Nucleofection Buffer"
Filter Pore Size: 0.2 µm
Liquid / Powder: Liquid
Quality: GMP
Size: 100 ml
Sterile: Yes
Storage: +2°C - +8°C
Downloads "Universal Electroporation / Nucleofection Buffer"