Panserin 293A, Serum-free medium for HEK-Cells in adherent culture, w: L-Glutamine

Panserin 293A is a complete ready to use medium for the serum-free cultivation of HEK293 cells (Human Embryonic Kidney) in adherent culture.

Composition

Based on DMEM additional trace elements, albumin, cholesterol, soy lipids, vitamins and hormones have been added to the medium.

Suitability and Applications

Panserin 293A is a particularly enriched medium optimized for the growth of HEK293 cells in adherent culture. HEK293 is frequently used for the expression of recombinant proteins and the proliferation of adenoviruses. Panserin 293A promotes a rapid attachment of the cells and guarantees high cell growth rates.

Application

Switching from serum-containing medium to Panserin 293A is often possible without special adaptation. For cell clones that do not tolerate such a simple switch to Panserin, we recommend cultivating the cells in Panserin with serum supplement and gradually reducing the serum content.

This procedure is supported by higher titers during seeding. The vitality status plays a crucial role for the successful transfer of cells into serum-free culture. Therefore, cells must be harvested during the logarithmic growth phase.

From our experience, cultivation from the stationary phase is significantly less likely to succeed. When transferring the cells, it must be ensured that after possible detachment with trypsin, the enzyme is removed by complete washing or inhibited by a trypsin inhibitor, as the neutralizing effect present in serum is omitted.