Panserin 701, Serum-free medium for Lymphocytes, w: L-Glutamine, w: PHA-L
€47.90*
Available, delivery time: 2-3 days
Product information "Panserin 701, Serum-free medium for Lymphocytes, w: L-Glutamine, w: PHA-L"
Add heparinized blood 1:1 with PBS and transfer to a centrifuge tube previously filled with lymphocyte separation medium (Pancoll density 1.077 g/ml): pipette carefully to avoid phase mixing!
Panserin 701 is a fully defined, complete ready-to-use serum-free medium for the cultivation of lymphocytes from whole blood.
Composition
Based on Iscove's MEM the medium is enriched with additional trace elements, albumin, cholesterol, lipids and vitamins. It contains the mitogen phytohemagglutinin (PHA) for a growth stimulation of lymphocytes.
Suitability and Applications
Panserin 701 has been developed for the serum-free cultivation of lymphocytes from whole blood. The herbal lectin (PHA) in Panserin 701 stimulates cell division.
Application
1. Isolation of lymphocytes from whole blood using density gradient centrifugation.
2. Cultivation and stimulation of lymphocytes.
- Resuspend lymphocytes in Panserin 701 at a cell density of 1 x 105.
- The phytohemagglutinin already contained in Panserin 701 stimulates the cell division of the lymphocytes.
- The incubation time is 48 to 72 hours, depending on the type and origin of the lymphocytes and further use.
- Further cultivation e.g. in Panserin 413. After approx. 14 days, restimulation with Panserin 701 is necessary.
| Cell Type: | Lymphocytes |
|---|---|
| Culture Type: | Suspension Culture |
| Liquid / Powder: | Liquid |
| Product Category: | Serum-Free Media |
| Size: | 100 ml |
| Sterile: | Yes |
| Storage: | -20°C |